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American Society of Microbiology ABMM Sample Questions:

1. A research laboratory is investigating the mechanism of action of a novel antiviral drug against herpes simplex virus. The drug is found to inhibit the viral DNA polymerase. Which of the following commercially available antiviral agents has a similar mechanism of action?

A) Oseltamivir
B) Amantadine
C) Acyclovir
D) Interferon-alpha

2. A researcher is studying the genetic relatedness of different isolates of Mycobacterium tuberculosis. Which of the following molecular typing methods provides the highest discriminatory power for distinguishing between closely related strains?

A) Spoligotyping
B) Pulsed-field gel electrophoresis (PFGE)
C) Restriction fragment length polymorphism (RFLP) analysis
D) Whole-genome sequencing (WGS)

3. A microbiology laboratory receives a cerebrospinal fluid (CSF) sample from a patient with suspected meningitis. Gram stain shows numerous Gram-negative diplococci. The organism grows on chocolate agar but not on blood agar without added CO2. The MOST likely identification is:

A) Listeria monocytogenes
B) Haemophilus influenzae
C) Neisseria meningitidis
D) Streptococcus pneumoniae

4. A Gram stain of anaerobic growth from a peritoneal fluid abscess reveals pleomorphic, pale-staining Gram- negative rods. The isolate grows on Bacteroides Bile Esculin (BBE) agar, hydrolyzing esculin (producing black colonies), and is resistant to kanamycin (1000 µg), vancomycin (5 µg), and colistin (10 µg) disks. Which organism group is most likely?

A) Prevotella melaninogenica group
B) Fusobacterium nucleatum
C) Clostridium perfringens
D) Bacteroides fragilis group

5. A research team is investigating the mechanism of bacterial resistance to a novel fluoroquinolone antibiotic.
They isolate a resistant strain of Escherichia coli from a patient with a urinary tract infection. Genetic analysis of this strain reveals a point mutation in the gyrA gene. This mutation MOST likely results in:

A) Modification of the ribosomal target site, preventing antibiotic binding.
B) Production of an enzyme that inactivates the fluoroquinolone molecule.
C) Alteration of the DNA gyrase enzyme, reducing its affinity for the fluoroquinolone.
D) Increased expression of efflux pumps that actively transport the antibiotic out of the cell.

Solutions: